CD300a Antikörper

Produktnummer ABIN238465

Dieser Maus Monoklonal Antikörper erkennt spezifisch CD300a in WB und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD300a Antikörper (ABIN238465)

Target: CD300a (CD300A)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD300a Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: MEM-260

Produktdetails anti-CD300a Antikörper

Spezifität: This antibody reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25 % of circulating T cells and 15 % of circulating B cells.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Produktmerkmale: Synonyms: CLM-8, CMRF35-H, CMRF35-H9, CMRF-35-H9, IRC1/IRC2, IRp60, CMRF35-like molecule 8,Inhibitory receptor protein 60, NK inhibitory receptor, CD300 antigen-like family member A

Aufreinigung: Protein A affinity chromatography.

Immunogen: HPB human acute lymphoid leukemia cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Western Blotting (Non-reducing conditions): Positive material: HPB cell lineNegative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD300a

Target: CD300a (CD300A)

Andere Bezeichnung: CD300A / CMRF35H

Hintergrund: CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.Synonyms: CD300 antigen-like family member A, CLM-8, CMRF-35-H9, CMRF35-H, CMRF35-H9, CMRF35-like molecule 8, IRC1/IRC2, IRp60, Inhibitory receptor protein 60, NK inhibitory receptor

Gen-ID: 11314

UniProt: Q9UGN4

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process